Composite
Pre A4

Part:BBa_K4060103

Designed by: CHEN YAN-ZHEN   Group: iGEM21_NYCU-Taipei   (2021-09-30)


[Pre A4] a pBad ligated before J23106

One of our goals is to create a better L-arabinose induced constitutive promoter. Here, we used a constitutive promoter (BioBrick_BBa_J23106) to co-regulate with pBad promoter (BBa_K206000). The pBad promoter is a positively regulated promoter, which will be inhibited by araC without the presence of L-arabinose. The L-arabinose can bind with araC and turn araC from a promoter inhibitor to a promoter inducer. In our kill switch design, we hope to generate a stronger pBad promoter with greater expression rate and wider inducing range. With the regulation of both constitutive promoter and L-arabinose induced pBad promoter, we hope it can help the pBad to increase its expression ability. In other words, we are trying to improve BioBrick_BBa_K206000. We want to ligate pBad promoter with a constitutive promoter to form a tandem promoter. We hypothesized that by adding a constitutive promoter beside pBad, the expression of the tandem promoter will be better than the normal pBad promoter (BioBrick_BBa_K206000). We compared the intensity of RFP gene constructed behind the pBad promoter and pBad-J23106 tandem promoter to see if the tandem promoter has better expression rate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 145
    Illegal NheI site found at 168
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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